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How To Guide: Nebula Genomics Whole Genome Sequencing

It’s easy to use your Nebula Genomics whole genome sequencing data with DNA analysis apps. After you obtain your Nebula files, navigating through the multitude of files in your Nebula account can be daunting. Each file contains a unique aspect of your genome, presenting a comprehensive but complex view of your genetic data.

Once your Nebula data is stored in your Sequencing account, One Genome technology automatically enhances your data by combining the highest quality DNA data from each of your DNA data files together into a single enhanced digital genome. This helps simplify analyzing and exploring your genome!

Import My Genome Data From Nebula Genomics

Automatically import your Nebula Genomics DNA data (Secure, Simple and Quick)

  1. Click the ‘Nebula Genomics’ button in our ⁠Upload Center.
  2. Follow the instructions and your import will begin.
  3. VCFs and PDFs are usually imported within an hour while FASTQ and CRAM files can take up to 48 hours before they appear in your account.
    • If your FASTQ and CRAM do not import, Nebula may have archived your files. See below for what to do if your files are archived.

Nebula archives FASTQ and CRAM files 14 days after they deliver these files to your Nebula account. Archived files first need to be unarchived before they can be imported.

We highly recommend importing your FASTQ and CRAM files in-order to obtain the best possible analysis. While it may take a bit longer, it is worthwhile to unarchive and import these files. 

How To Unarchive Your Nebula Files

  1. Sign in to your Nebula account and go to the ⁠download page.
  2. Click the button below the FASTQ files and the CRAM files to unarchive them.
  3. Wait 48 hours after unarchiving your FASTQ and CRAM files. This is the time Nebula requires to unarchive your files so they can be downloaded.
  4. Return to Sequencing’s Upload Center and click the Nebula Genomics button to start the automatic import.

Download your Nebula data and upload the data to your Sequencing account

  1. Sign in to your Nebula Genomics account using ⁠this link, which will take you directly to the page to download your data.
  2. Download the files for each of your kits.
    • While we recommend obtaining all of your DNA data files, due to their size this can take a while. You may want to start with your FASTQ files as these are the most important to obtain.
    • If FASTQ and CRAM files are archived, please follow the instructions above for unarchiving the files.
  3. After downloading your files, sign into Big Yotta, our secure uploader for large files.
    • Sign in using your account credentials.
  4. Upload your Nebula files using Big Yotta.

Nebula Genomics 0.4x vs 30x vs 100x

Sequencing’s platform is optimized for 30x whole genome sequencing (WGS). All files from 30x sequencing are compatible with our DNA analysis apps and reports.

After analyzing files from 0.4x WGS, our bioinformatics team has determined the quality of the data is not optimal for many of the health-related apps and reports, such as the Next-Gen Disease Screen. Because of this, 0.4x files are are no longer compatible with our platform and cannot be used with the apps and reports in our marketplace. Learn more about the difference between 30x and 0.4x WGS

If you’ve had 100x WGS, the genome VCF file can be uploaded and will be compatible with our apps and reports. The FASTQ and CRAM files, however, should not be uploaded because they will not be compatible.

Indexing Files

Files that end in .tbi are known as an index file. They do not provide any additional information beyond the data included in the FASTQ files and CRAM file. Because they don’t provide any additional value, TBI index files are not compatible with our platform. While you can upload them to your Sequencing account, they will be stored in the ‘Incompatible Files’ folder in your My DNA Data page.

Improve your Nebula Data with One Genome Technology

One Genome is a new technology that automatically combines the highest quality DNA data from each of your DNA data files together into a single enhanced virtual genome.

Obtain a single, enhanced virtual genome

If you have DNA data from multiple test providers, such as Nebula Genomics, 23andMe and AncestryDNA, One Genome will use the highest quality data from each file to generate your genome.

While WGS data is far more comprehensive than genotyping tests, WGS data will always have some low-quality calls. Data from other tests you’ve taken, such as a 23andMe test, may then be used in place of a low-quality WGS call. This helps ensure your virtual genome is as complete as possible.

Ensure your genome contains the most up-to-date data on all genetic variations

One Genome Technology is especially powerful if you have your FASTQ or BAM files stored in your Sequencing account. One Genome processes will process your FASTQ or BAM (or both) using a highly advanced pipeline so that your virtual genome always contains the most comprehensive, up-to-date data on all genetic variations.

This includes aligning your genome to the current reference genome as well as generating data for all genetic variations (SNP, INDEL, CNV, SV and MITO), all aligneed GRCh38 (the latest reference genome).

Whenever there’s an update to the pipeline, your virtual genome will be automatically re-generated. This ensures your enhanced virtual genome is always up-to-date.

Apps analyze your genome instead of individual data files

Once One Genome is applied to your account and your enhanced virtual genome is generated, you’ll be able to use DNA analysis apps simply by selecting your genome. Instead of having to decide which file is best to use with an app (and potentially using an app multiple times to obtain full analysis of all variations in your genome), when you start an app with your virtual genome, the app will automatically process your entire genome.

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Nebula Genomics is a registered trademark of Nebula Genomics, Inc. The use of the name and logo are for compatibility information only and does not imply approval or endorsement of by Nebula Genomics, Inc.

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